When AKTA protein purification instrument reports a pressure too high alarm during operation, you can follow these common troubleshooting steps: 1. **Flow rate check**: Confirm if the set flow rate exceeds the maximum tolerable flow rate of the chromatography column. Adjust it to the reasonable range if it does. 2. **Column inspection**: Check the chromatography column for uneven filler packing, internal bubbles, or blockages; rebalance or repack the column as needed. 3. **Flow path blockage check**: Inspect pipelines, mixing pools, and other components for blockages; clean or replace blocked parts. 4. **Buffer verification**: Ensure buffers are fully degassed, bubble-free, and uniformly prepared; filter samples/buffers if there are unfiltered impurities. 5. **Pressure sensor check**: Verify the pressure sensor's normal operation; calibrate or replace it if malfunctioning. If you encounter difficulties in troubleshooting or need professional support, Jiangxi Bio provides comprehensive solutions for AKTA and other brands (Thermofisher, Waters, Agilent, GE, Cytiva, Beckman) including repair, leasing, cleaning maintenance, consumables/accessories supply, and chromatography application consulting. Our team can offer efficient, professional services to resolve your instrument issues.