For Agilent 1100 HPLC pump pressure anomalies, you can troubleshoot and resolve them with the following steps: 1. **Check Purge Valve Filter**: Open the Purge valve, use pure water as mobile phase at 5mL/min flow rate. If pressure exceeds 10bar, replace the PTFE filter (filter白头). 2. **Inspect Plunger Seals & Valves**: Pressure fluctuations, abnormal rise/drop, or retention time drift often indicate worn plunger seals or valve failures. Regularly check and replace worn seals (ensure material compatibility with solvents). Annual maintenance of plunger rod assemblies is recommended. 3. **Maintain Outlet Ball Valve**: Disassemble and clean the outlet ball valve, check for worn gaskets and replace if needed. 4. **Prevent Salt Crystallization**: For quaternary pumps, connect salt solutions to lower ports and organic solvents to upper ports of the proportion valve. Flush all proportion valve channels with water regularly to remove salt deposits. 5. **Check for Leaks**: For low flow rates (<=0.2mL/min), inspect all joints for leaks using pressure or leak tests. 6. **Maintain Degasser**: Flush the vacuum degasser with at least 3x its volume (30mL for standard,10mL for micro) before use if idle (e.g., overnight) or using volatile solvents. 7. **Drain Valve Filter Check**: Similar to Purge valve, if pressure exceeds10bar at5mL/min water flow, replace the drain valve filter. To ensure your Agilent 1100 HPLC system operates stably and minimizes downtime, **匠析生物** provides professional services tailored to your needs: We offer comprehensive solutions including repair, regular maintenance (such as seal replacement, filter change, and system cleaning), supply of original and compatible spare parts (seals, filters, valves for Agilent and other brands like Thermofisher, Waters, GE), and chromatography application consulting. Our team can help with preventive maintenance plans to avoid pressure anomalies and other common issues, ensuring your experiments proceed smoothly. If you have any needs related to instrument maintenance or repair, feel free to contact us for support.