For Agilent HPLC pump pressure instability, you can try the following troubleshooting steps: 1. Check liquid flow: Open the purge valve, set the flow rate to 1-2ml/min, start the pump, observe if there is stable flow (no backflow; use a graduated cylinder to verify flow accuracy). 2. Inspect for leaks: Check pipelines connected after the pump for leaks, as leaks often cause unstable pressure. 3. Maintain the purge valve: If the pressure exceeds 10bar when purging with pure water at 5mL/min, replace the PTFE filter inside the purge valve. 4. Perform regular pump maintenance: Replace pump heads as needed; for systems with plunger rod cleaning function, enable and use it daily. 5. Balance the chromatographic system properly using appropriate methods. If these steps do not resolve the issue, or if you need professional and reliable maintenance services, Jiangxi Bio (匠析生物) can provide comprehensive solutions. We specialize in after-sales repair and maintenance for Agilent and other top brands (including Thermofisher, Waters, GE, Cytiva, Beckman) of laboratory instruments such as HPLC, MS, chromatography systems, etc. Our services cover repair, rental, cleaning and maintenance, and we also supply original or high-quality accessories and consumables. Additionally, our chromatographic application consulting team can help optimize your experimental processes. Feel free to contact us for your instrument maintenance and application needs.