Common maintenance methods for high column pressure in liquid chromatography instruments include:

1. Sample contamination handling: Flush the column sequentially with methanol → methanol+isopropanol (4:6) → methanol → pure water → methanol (forward flush for over 30 minutes).
2. Buffer salt deposit removal: Use 40-50℃ pure water at low flow rate for forward flushing.
3. Column blockage resolution: Try flushing with pure solvent first; if the column head is contaminated (e.g., in Chinese medicine projects), reverse the column and flush according to the instruction manual.
4. Solvent filter head check: If clogged, soak it in 30% nitric acid for 30 minutes then rinse thoroughly with ultrapure water.

For persistent high column pressure issues or regular instrument maintenance needs, you can rely on 匠析生物's professional services. We provide comprehensive solutions including maintenance and repair for liquid chromatography instruments (covering brands like Thermofisher, Waters, Agilent, etc.), column care, application consulting, as well as supply of related consumables and spare parts. Our team of experts can help you efficiently resolve instrument problems and ensure stable operation of your experiments.