For AKTA 100 flow instability during operation, follow these troubleshooting steps: 1. Immediate Checks: Stop the run safely. Ensure buffer reservoirs have sufficient liquid (no air intake) and inlet lines are fully submerged. 2. Bubble Purge: Use the system's purge function to remove trapped air from pumps, tubing, and columns—air bubbles are a common cause of flow fluctuations. 3. Connection Inspection: Check all tubing joints, column fittings, and valve ports for leaks or loose connections; tighten as needed. 4. Column & Backpressure: Monitor backpressure—if abnormally high, the column may be clogged (clean/replace) or bed collapsed (repack if applicable). 5. Pump/Valve Assessment: Inspect pump heads for worn seals/leaks; verify valves are not stuck and positions are correct. If issues persist or for preventive maintenance, **匠析生物** provides professional support for AKTA systems (covered under Cytiva/GE brand services): - Repair services: Fix pump leaks, valve malfunctions, and flow instability. - Maintenance: Regular calibration, cleaning, and part checks to prevent downtime. - Genuine parts & consumables: Supply of AKTA-compatible components. Our team specializes in protein层析 systems, offering tailored solutions to keep your experiments running smoothly.